For molecules with non-trivial point group symmetry, the Fourier projection theorem implies that each 2D projection image corresponds to multiple slices, where the number of slices equals the size of the symmetry group. As a consequence, determining the 3D structure requires fewer images for molecules with larger point-group symmetries. At the moment, our algorithms for orientation assignment do not support non-trivial symmetry groups, a functionality that we plan to add in the future.